At the end of the day it doesn’t matter so much if they’re in 2x 2 bays or 1x 4 bay that’s backing itself up. It might give a little extra redundancy and safety to have them on separate NAS but the backup software is what’s going to be doing the heavy lifting here and it shouldn’t really matter whether it’s talking to two different disks/arrays on the same machine/NAS (as long as the NAS allows you to split the 4 drives into 2 different arrays which from my experience they do)
For RAID that’s pretty much it as far as I know, but I’m pretty sure it can be a lot simpler and more flexible using some of these newfangled filesystems that are out nowadays like LVM and ZFS and maybe BTRFS? I can’t pretend I’m super up to date on all the latest technologies, I know they can do some really incredible stuff though. I’m not familiar enough to recommend it, but it might be worth looking into what they can do for you if your NAS supports it. From what I understand they don’t use RAID at all, although they might be able to simulate it, instead they treat disks as JBOD (just a bunch of disks) and use their own strategies to spread whole filesystems and partition structures across them in various safe and redundant ways that are way more flexible, that don’t care about disk size or anything like that, they’ll handle any shapes and sizes and I think they can be expanded and contracted pretty freely. I think ZFS in particular is really heavily used for this and supports some crazy complicated structures.